Unique biodiversity of the Red Sea brine floor revealed by comparative taxonomic analysis of Atlantis II and Discovery Deep brine sediments

The sea floor is a unique and diverse ecosystem to investigate how geochemical processes affect the diversity of biological life. Red Sea brine pools are water bodies characterized by a unique multitude of extreme conditions, including high temperature, high salinity, and unusual high concentration of heavy metals. We performed a comparative metagenomic analysis of the microbial communities in the sea floor of the Atlantis II (2168 m; 68oC), Discovery Deep (2166 m; 48oC) brine pools and two non-brinesites (1,856 m; 21.93°C and 1,937 m; 31.87°C) in the Red Sea. Each brine core wasvertically dissected into seven distinct subsections. CHN&S profiles showed significant fluctuations in sulfur and nitrogen levels in two of the brine pool subsections. A comprehensive 16S rDNA analysis of about one million 16S rDNA reads, allowed the identification of an exclusive assemblage of microbial communities in the sulfur rich Atlantis II and the nitrogen rich Discovery Deep brine pool subsections when compared to adjacent brine and non-brine sediments. Proteobacteria, Actinobacteria, Cyanobacteria,and Deferribacteres were the most abundant bacterial phyla. Interestingly, the S-rich Atlantis II subsection showed distinctive abundance of the recently identified Chloroflexiorder Anaerolineales, Fusobacteria and OP1. Crenarchaeota dominated all the sediment samples with the exception to the S-rich Atlantis II and the N-rich Discovery Deep sample that was dominated by Euryarchaeota. Our study illustrates a distinct stratification of the microbial communities in both the nitrogen rich and the sulfur rich brine subsections. However, the lack of apparent stratification in the remaining layers of the brine and non-brine sediments despite variations in metals and CHN&S profiles implies that sulfur and nitrogen are the major players that dictate the microbial assemblage in the Red Sea

The microbiome of The Egyptian Red Sea proper and Gulf of Aqaba

The Red Sea is one of the most unique environments worldwide. It possesses a unique geography, physical, chemical and biological characteristics. It encounters several ecosystems articulating with each other, these include, corals, mangroves, algae, fisheries, invertebrates and microbiota of each one of these along with microbiota of the Red Sea waters and sediments. Studying the collective microbial communities of the Egyptian Red Sea coastal sediments have not been reported before. In regards to the severe pollution impacting the different Red Sea ecosystems, sediments samples have been collected from different impacted sites. The selected sites included 1- four ports for shipping aluminum, ilmenite and phosphate, 2-a site previously reported to have suffered extensive oil spills, 3-a reported tourism impacted site 4- two mangrove sites and 5-two lakes. Bacterial communities for each site have been studied through two different approaches, Culture-Dependent and Culture-Independent approaches. Pyrosequencing of V6-V4 hypervariable regions of 16S rDNA, isolated through the two approaches, has been used to assess the microbial community of each site. Physical parameters, Chemical analysis for 29 elements, selected semi-volatile oil contents, along with Carbon, Hydrogen, Nitrogen and Sulfur (CHNS) contents have been measured for each site. 131,402 and 136,314 significant reads have been generated through the Culture-Dependent and Independent approaches, respectively. Generally, Proteobacteria, Firmicutes, Actinobateria, Fusobacteria, Gemmatimonadetes and Bacteriodetes are the major bacterial groups detected through the two approaches. The Culture-Dependent datasets distinctive analysis revealed three main patterns (1) marine Vibrio spp.-suggesting a marine Vibrio phenomenon ; (2) potential human pathogens; and (3) oil-degrading bacteria. While the Culture-Independent datasets analysis reported (1) an Egyptian Red Sea Coastal Microbiome, taxa detected in all the sites and (2) Hydrocarbon biodegrading bacteria predominance to the majority of the sites; particularly in two ports. On the other hand, the two lakes, through the two approaches, showed unique bacterial patterns, which generally grouped into anaerobic, halophilic and sulfur metabolizing bacteria. Individually, sites showed unique evolution of their microbial communities based on minor intrinsic and imposed variation per sites. Our results draw attention to the effects of different sources of pollution on the Red Sea and suggest the need for further analysis to overcome the hazardous effects observed at the impacted sites

Classifying Web 2.0 Supported Applications By Pattern Of Usage: Functional & Technical ISSUES

The rapid evolution of Internet technologies have witnessed new Web elements, such as blogs, wikis, social networking, social bookmarking, and other related applications referred to as Web 2.0. Web 1.0 paradigm was related with passive, just receptive users, whereas Web 2.0 paradigm relies mainly on user participation and user-generated content. In Web 2.0 applications users are invited to comment, share, edit, classify, as well as remix data from multiple sources. Although there are several Web 2.0 applications in the market there is still lack of a profound approach guiding the analysis, design and development of such applications. This paper suggests classifying Web 2.0 tools by “Pattern of Usage” or in other words the functionalities that characterize their specific features. By reviewing several literatures we extracted multiple attributes related to functionalities of Web 2.0 tools. These have been crystallised into 7 patterns of usage that include; Inter-connectivity, Content authoring, Content tagging & rating, Content aggregation & syndication, Content remixing, Content streaming and File sharing. By interlinking functionality/ usage with underlying technologies, techniques and architecture we provided insight into design and technical requirements for Web 2.0 supported applications. Furthermore we broke down the patterns into basic, elementary to include Inter-connectivity, File sharing and Content remixing, and secondary, supportive to include the other four patterns. This would provide the technical core for any development methodology targeted at Web 2.0 applications

CONDA-PM -- A Systematic Review and Framework for Concept Drift Analysis in Process Mining

Business processes evolve over time to adapt to changing business environments. This requires continuous monitoring of business processes to gain insights into whether they conform to the intended design or deviate from it. The situation when a business process changes while being analysed is denoted as Concept Drift. Its analysis is concerned with studying how a business process changes, in terms of detecting and localising changes and studying the effects of the latter. Concept drift analysis is crucial to enable early detection and management of changes, that is, whether to promote a change to become part of an improved process, or to reject the change and make decisions to mitigate its effects. Despite its importance, there exists no comprehensive framework for analysing concept drift types, affected process perspectives, and granularity levels of a business process. This article proposes the CONcept Drift Analysis in Process Mining (CONDA-PM) framework describing phases and requirements of a concept drift analysis approach. CONDA-PM was derived from a Systematic Literature Review (SLR) of current approaches analysing concept drift. We apply the CONDA-PM framework on current approaches to concept drift analysis and evaluate their maturity. Applying CONDA-PM framework highlights areas where research is needed to complement existing efforts.Comment: 45 pages, 11 tables, 13 figure

Evaluating Tourism Service Quality Provided to the European Tourist “Applied on the British tourist"

Abstract Service quality (SERVQUAL) has received a considerable attention in the last 50 years. While Service quality are well documented in the literature review, fewer studies have been investigates the relationship of Service quality with customer satisfaction and behaviour intention, and particularly in tourism. The aim of this paper is to examine the causal direct and indirect impact of service quality on customer satisfaction and behaviour intention. A total of 390 usable responses were obtained with a response rate of 71%. Structural equation modelling was used to analysis the current research data. The structural equation modelling results indicate that all the employed dimension to measure service quality (tangibility, reliability, responsiveness, assurance, and empathy) have direct influence on customer satisfaction and indirect impact on behaviour intention through customer satisfaction. These results help to clarify the mixed findings in the literature concerning the pattern of the causal relationship between service quality with customer satisfaction and behaviour intention. Finally, conclusions and limitations are outlined

Mobilizing International Law in the Palestinian Struggle for Justice

Those involved in mobilizing international law to achieve justice for the Palestinians have invoked numerous legal and governance institutions, at both international and national levels. For various reasons, international law has understandably been regarded with a high level of skepticism by many Palestinians, particularly from legitimacy and effectiveness standpoints. However, law has also ignited the Palestinian civic imagination and has led to bold and creative initiatives, including efforts to hold both states and (corporate) non-state actors accountable through legal and other means, and even to construct alternative models for nation building. This introduction to a Special Issue of the Global Jurist on ‘International Law and the State of Israel’ emerges from an international conference that took place at Cork City Hall and at the campus of University College Cork in Ireland in March 2017. Our message for producing this Special Issue and indeed for our colleagues who organized the conference in the first place was simple: while we cannot afford to neglect law in envisioning alternative futures in Israel/Palestine (including statehood), justice always remains a guide

Molecular and cellular effects of gold nanoparticles treatment in experimental diabetic myopathy.

Background This study aims to address the effects of gold nanoparticles (AuNPs) on diabetic myopathy in streptozotocin (STZ)-induced diabetic rats. Materials and methods Adult male rats were separated into three groups (n = 15): non-diabetic control (ND), diabetic (D), and diabetic treated with AuNPs (2.5 mg/kg, D + AuNPs) intraperitoneally for 4 weeks. A single injection of 50 mg/kg STZ was used to induce diabetes. Results Treatment with AuNPs lowered blood glucose levels. Skeletal muscle mRNA expression of two muscle-specific E3 ubiquitin-ligases enzymes, F-box-only protein 32 (FBXO32) and muscle RING-finger protein-1 (MuRF1) were upregulated in the D group. Diabetic rats showed significant increases in the skeletal muscle expression levels of plasminogen activator inhibitor-1 (PAI-1), tumor necrosis factor-α (TNF-α), transforming growth factor-β1 (TGF-β1), and a decrease in glucose transporter 4 (GLUT4) expression. Superoxide dismutase (SOD) activity decreased and malondialdehyde (MDA) level increased in skeletal muscles of D group. Compared to the D group, expression levels of FBXO32, MuRF1, PAI-1 TNF-α, and TGF-β1 were decreased in the D + AuNPs group, and mRNA of GLUT4 increased. Furthermore, in D + AuNPs group, skeletal muscle MDA levels decreased while SOD activity increased. Conclusion In experimental models, AuNPs can ameliorate muscle atrophy by reducing hyperglycemia, inflammation, and oxidative stress, and by suppressing the ubiquitin-proteasome proteolytic process